Peptide Reconstitution Calculator
Free UK peptide reconstitution calculator. Work out the concentration of a reconstituted vial and the volume that holds a given amount, in mL and graduation marks. Volumetric conversion only.
Choose your syringe size, vial strength and the volume of solvent added. The calculator converts a target amount into mL and graduation marks.
Syringe capacity
Total barrel volume
Vial strength
Lyophilised peptide in the vial
Bacteriostatic water added
Total reconstitution volume
Target amount per measured volume
Amount of peptide in each measured volume
Measured volume
Volume holding the target amount
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Concentration
After reconstitution
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Measurements per vial
At this quantity
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Graduation mark
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Volumetric conversion only. Figures assume a syringe graduated at 100 units per mL.
Supplied strictly for in-vitro laboratory research use only.
Need a solvent? Bacteriostatic water 10ml, £5.99
0.9% benzyl alcohol, the standard solvent for reconstituting lyophilised research peptides. UK stock.
How the calculation works
The calculator does three steps of arithmetic. Nothing else goes into the result.
- Concentration = peptide in the vial ÷ solvent added. 10 mg in 2 mL gives 5 mg/mL, which is 5,000 mcg/mL (1 mg = 1,000 mcg).
- Volume = target amount ÷ concentration. 500 mcg at 5,000 mcg/mL is 0.1 mL.
- Graduation mark = volume × 100, on a scale with 100 marks per mL. 0.1 mL is the 10 mark.
Adding more solvent lowers the concentration, so each mark holds less peptide. Adding less raises it.
Common vial and solvent combinations
| Vial | Solvent added | Concentration | Peptide in 0.1 mL (10 marks) |
|---|---|---|---|
| 5 mg | 1 mL | 5 mg/mL | 500 mcg |
| 5 mg | 2 mL | 2.5 mg/mL | 250 mcg |
| 10 mg | 1 mL | 10 mg/mL | 1,000 mcg |
| 10 mg | 2 mL | 5 mg/mL | 500 mcg |
| 10 mg | 3 mL | 3.33 mg/mL | 333 mcg |
| 20 mg | 2 mL | 10 mg/mL | 1,000 mcg |
| 20 mg | 3 mL | 6.67 mg/mL | 667 mcg |
mg and mcg converter
1 milligram (mg) is 1,000 micrograms (mcg).
Questions about reconstitution
Why bacteriostatic water rather than sterile water?
Bacteriostatic water contains 0.9% benzyl alcohol, which suppresses bacterial growth so a vial can be entered more than once over a working period. Plain sterile water has no preservative, so a solution made with it is normally treated as single-use. Our solvent guide covers the cases where a different solvent is the better choice.
How should the solvent be added?
Let the vial reach room temperature, then add the solvent slowly down the inside wall of the vial rather than onto the powder. Swirl gently until dissolved. Do not shake or vortex: foaming is a sign of peptide denaturing at the air–liquid surface. The reconstitution and storage guide has the full method.
How long does a reconstituted solution keep?
Store it refrigerated at 4°C, protected from light, and avoid repeated freeze–thaw cycles. Each product page lists the handling conditions for that compound. See our storage guide for more detail.
What are "units" in the result?
They are graduation marks on a scale of 100 marks per mL, so 1 unit is 0.01 mL. If your measuring syringe is marked differently, use the mL figure instead.
Does the calculator say how much to use?
No. It converts amounts and volumes, nothing more. MMpeptides does not provide usage or dosing guidance. All products are supplied strictly for in vitro laboratory research and are not for human or veterinary use.